The Effect of Hydrogel Extract of Kirinyuh Leaf On 2nd Degree Burn on Wistar Rat with Regard to Angiogenesis and Fibroblast Amount

Abstract

Burn injuries continue to pose a significant health concern for society, as they can lead to permanent impairment of body functions and appearance, as well as disrupt daily life, including future employment prospects. This research aims to evaluate the potential of kirinyuh leaf hydrogel extract as a topical therapeutic agent in accelerating the healing of second-degree burn wounds in Wistar rats. The parameters assessed include the expression of VEGF, the quantity of neovascularization, and fibroblast count in the burn wounds. This study utilized an experimental design with a Post-Test Only Control Group, administering 5%, 10%, and 15% kirinyuh leaf hydrogel extract to Wistar rats induced with second-degree burns. Data normality was then analyzed using the Shapiro-Wilk test, followed by a One-Way ANOVA test. The results revealed a significant increase in VEGF expression, neovascularization, and fibroblast count when using kirinyuh leaf hydrogel extract compared to the negative control group, with the most significant effect observed at a concentration of 15% (p<0.05).